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KMID : 0545119960060050352
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Journal of Microbiology and Biotechnology
1996 Volume.6 No. 5 p.352 ~ p.357
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Salting-out Effects on the Partition of Proteins in Aqueous Two-phase Systems
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KIM CHAN-WHA
RHA CHO-KYUN
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Abstract
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The partition of proteins in the salt-rich phase of polyethylene glycol (PEG)/salt aqueous two-phase systems is limited by the salting-out effects of salt. The logarithm of the concentration of proteins partitioned in the salt-rich phase decreases linearly with increases in the concentration of salt in the salt-rich phase (salting-out). Therefore, the partition of a given protein in the salt-rich phase of aqueous two-phase systems can be estimated from the salting-out constant. The slope of the solubility line (salting-out constant) for a given protein is determined by the type of salt in the two-phase systems.
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